After consuming the seven Skullgirl candidates (Filia, Cerebella, Peacock, Parasoul, Ms. Fortune, Painwheel, and Valentine) into herself, Double gave the Skull Heart to the new chosen one which it turns to be Marie's clone daughter Kira. She had contact her masters to tell them that she found the new Skullgirl and the Trinity had created a soldier to help Double out. Double was infatuated with the soldier named Prelate who appears to look like sleek, silver metallic being with few defining features aside from his darker blue-colored stripes and eyes as if he was more robot-like. Double and Prelate were falling in love with each other and the two have a daughter named Charlotte. They both taught Charlotte everything about her service to the Trinity and the new Skullgirl. Assissting Laura, Double and Prelate must make that their daughter won't betray her masters.
Years later, Charlotte is now an ageless formless monster working with her masters Aeon and Venus on their video game that showed images of eight new girls on the screen.
"Well, well, well." Aeon spoke with an mischeivous grin. "New girls to take. They are the daughters of the Skullgirl Candidates. I've used my powers to prevent them girls from being erased."
"Sweet!" Venus said, clapping her hands. She then turned to around to face Charlotte who is feeling emotionless. "Charlotte! I think it's time to give you a mission to fulifull."
"And that is?" Charlotte asked.
"Find seven girls that is worthy for the Skull Heart and her her carry our wills when Kira finshes the job." Venus replied. "Unlike Marie, who betrayed us."
"And you won't let any those seven girls nor Kira betray us, got it?" Aeon stated.
Charlotte bowed her head. "If it's the right thing to do, I'll do it." Then, she disappears.
At the Divine Trinity, Charlotte spotted her mother confronting the new silver-haired girl wearing her dark green tight dress and knee-length boots feeling depressed and lonely. Charlotte could feel the girls' feelings towards Anti-Skullgirls Lab were extremely hatred and painful. She carefully watched her mother Double handing a mysterious artifact the girl: the Skull Heart.
"You don't have to run anymore, child." Double spoke. "The Trinity had heard your cries, Kira. Take it."
Once Kira touched the Skull Heart and instantly transformed into the Skullgirl because of her impure heart she carries, Double faced her daughter with her usual praying stance.
"Charlotte." Double spoke again. "I want you to collect the daughters of the Skullgirl candidates for our masters immediately."
"As you wish, mother." Charlotte replied as she left the Divine Trinity.
