After wishing to becomes the new Skullgirl for the arrival of Painwheel, Valentine has used the Skull Heart's power to revive her friends from the Last Hope back to life to let them performed more advanced scientific research. Valentine later fall in love with a male Lab Zero's scientist named Kale and the two have quintlepts named Cissy, Roxanna, Lenora, Maylena, and Laura. Later on, she had battle Painwheel and was defeated as she told her husband to take care of their daughters and not let them know about her before she disappears. The Skull Heart quickly faded away as if it went back to the Trinity.

Years later, Laura is now an beautiful twenty-six year old nurse working on the parasities with her sisters. But unforetunaley, the Skullgirl and her aid Charlotte appeared and attacked the Last Hope, killing the four sisters in the process. The Skullgirl had conjure up a scissor made of bone and was ready to cut Laura's neck off. Laura felt that her life is doom, but then realized something that will stop her.

"Wait!" Laura stopped the Skullgirl's tracks. "I can help you! Just give me a chance! I could even destory the Anti-Skullgirls Lab for ruining your mother! Just please don't kill me!"

The Skullgirl was thinking about the nurse's comment and dropped her bone scissors down to the ground as it vanished in thin air.

"Alright." The Skullgirl calmly spoke. "What do you think, Charlotte?"

The young nun examined the nurse carefully and face back at the Skullgirl. "She's useful. After all, I do want a personal friend."

Sorry, sisters. Laura thought with a tear on her left eye. I don't have a choice, but I will avenge you all from the Skullgirl.

At the Grand Cathedral of the Divine Trinity, Laura was confident to serve the Skullgirl but she wants her new project to finish her up that she couldn't do herself. She's aware that Charlotte must not find out her true intentions.

"Laura, don't let Lab 8 interfere with my plan." The Skullgirl ordered. "Find them and destory them."

"As your wish, Kira." Laura said with a bow. "I'll leave immediately."