Prologue:

In 1995, Quinn Mallory, Wade Welles, Professor Maximillian Arturo and Rembrandt "The Cryin Man" Brown disappeared without a trace from Quinn's basement laboratory. Nearly two decades later, Michael Josephson, a college student who discovered Quinn's notes, attempted to replicate the experiment along with his friend Sam Evans, traveling to another world and meeting Rebecca Collins. They ended up lost like Quinn when a shockwave shorted out the timer's motherboard, erasing their and Rebecca's home coordinates. The trio spent their time escaping from danger, losing and regaining the timer and hoping that eventually they'd find their way home. On the way they met Quinn and his group of sliders, which now included his brother Colin and Captain Maggie Beckett, an army officer they had met on their journey.

The trio's quest continued, but was derailed when Michael realized they were being tracked by the evil Kromagg general Tiberius, along with Michael's evil double, and Michael abandoned Sam and Rebecca to protect them, escaping the Kromaggs and ending up on Earth Prime. After rebuilding the timer, he went back to find Sam and Rebecca, but they got captured by the Kromaggs and detained, learning about a planned invasion of Earth Prime, and stealing a Manta ship, they give Earth Prime a fighting chance, and free Michael's double, who adopts the name James to differentiate themselves, helps them and returns to his own world to save it from the Kromaggs. Eventually, with the help of The Doctor, an alien with a time machine, they foil Tiberius once again and locate Rebecca's home coordinates and go there to return her home, but due to a case of mistaken intentions, they are arrested and Rebecca is forced to abandon her home once again.

Following that, they continue their journey, until they end up separated, with Rebecca reuniting with James, and Michael and Sam on trial for alleged crimes against the multiverse. During the trial, Quinn and his group end up landing there too, along with Rebecca and the trial is revealed to be happening in a simulation, run by Logan St. Clair, an old foe from Quinn's past. Logan, a female double of Quinn, was thought to be dead, but had instead been sent to the past of the Kromagg homeworld, putting the entire Kromagg-Human War into action as an attempt to destroy Quinn, revealing that nearly every problem that the original Sliders ran into was a result of Logan's revenge plans, the Kromaggs, Arturo's death, Wade being sent to the breeding camp, Quinn's apparent merging with Mallory and Colin becoming "unstuck", were all attempts to get led back to Earth Prime, so Logan could take it over, as a final piece of revenge. They escape imprisonment and Quinn, Maggie and Michael go to raise an army and send the rest of the group home.

They all meet up again, as Quinn formulates a plan to stop the Kromaggs for good. Combining his double's slidewave machine, Geiger's Combine and the Slidecage, he plans to use those to isolate all the Kromaggs onto a world that is impossible to escape from. They work on and go to head Kromagg outpost to activate the machine and destroy the Kromaggs once and for all. They succeed in stopping Logan and activate the device, causing all the Kromaggs on the outpost, and throughout the multiverse to get sucked into a world where they will be contained. However, the device's heavy strain on the multiverse causes all their timers to start timing out, and Quinn and the others leave first, leaving just Michael, Sam and Rebecca behind, as their timer had a few minutes on it's timer. As they stand there, the timer was shot out of Michael's hands, revealing Tiberius had survived the slidewave as he had been close to the epicenter of the blast radius, and was untouched.

Tiberius takes Rebecca hostage and forces Michael and Sam to reverse the wave, they comply, but before he can react, they shoot him with the device and causes him to become unstuck, meaning he would not be dead, but be sent hurtling through the multiverse without a permanent physical form, however that fact is not known to the trio. Their timer is glitching as it times out, so they are unsure if they should use it, but as the world they are on might soon become unable to slide to or from, they slide anyway, ending up on the first world they landed on together, without their home coordinates, putting them back where it all began. Their sliding adventures continue, with them finding new friends, new enemies and countless more worlds before they can return home.