Hamato Kyle: First born son of Hamato Yoshi and Tang Shen, brother of John, Luke, Karl, Miwa, Leonardo, Raphael, Michelangelo, Donatello, owner of Dogpound and Rahzar, boyfriend of Gwen Stacy, he once mutated into a three headed cobra named Ssserpent.

Hamato John: Second born son of Hamato Yoshi and Tang Shen, brother of Kyle, Luke, Karl, Miwa, Leonardo, Raphael, Michelangelo, Donatello, owner of Fishface, Slash 2.0, boyfriend of Mary Jane Watson.

Hamato Luke: Third born son of Hamato Yoshi and Tang Shen, Brother of Kyle, John, Karl, Miwa, Leonardo, Raphael Michelangelo, Donatello, owner of Spy-Roach, Rockwell, Boyfriend of Felicia Hardy.

Hamato Karl: Fourth Born son of Hamato Yoshi and Tang Shen, brother of Kyle, John, Luke, Miwa, Leonardo, Raphael, Michelangelo, Donatello, owner of Ice Cream Kitty, Tokka, Boyfriend of Selena Kyle.

Hamato Miwa: Fifth born and first daughter of Hamato Yoshi and Tang Shen, brother of Kyle, John Luke, Karl, Leonardo, Raphael, Michelangelo, Donatello, Owner of Klunk and Snakeweed, girlfriend of DR Chaplin.

Tiger Claw: Hamato Yoshi's brother, uncle to the Hamato Children, Father of Lotus Blossom.

Newtralizer: Ally to the turtles.

Slash: Similar to 2012 version.

Dogpound: Pet to Kyle, ally to the turtles.

Rahzar: Pet to Kyle, former enemy of the turtles, now ally.

Tokka: Pet to Karl, former enemy of the turtles, now ally.

Anton Zeck: One of three best friends of Kyle and was mutated into a warthog named Bebop, and is African American.

Ivan Steranko: One of Kyle's three best friends and is Russian, mutated into a rhino named Rocksteady.

Martin: One of Kyle's three best friends, is an ally to the turtles.