While most flaming flying skeletons set off to complete their mission for their master, only eight of them has sensed eight girls that has strong, powerful aura on them. Each of the skeletons flew down to sense the girls' directions in order to get them to their master. Just before each girls could attack or react, the skeletons grabbed them by the throat and locked their eyes with the girls', making them feel woozy and allowed them to become unconscious. When the skeletons encased the girls in their ribs, the Last Hope were on the case. Once they saved them, including their teammate, the eight girls were still knocked out and was taken to the Anti-Skullgirls Lab.

Alice woke up to found herself onto muscular android's arms. Before she could ask him where he's taking her, the entrance door in front of them was open and noticed that seven other girls were sitting on the black half circular couch. As she stared cautiously at the fellow girl, Dr. Adel stepped into the room from the same entrance his fellow android had walked through and approached the eight girl with a warm smile.

"Good evening, ladies." Dr. Adel greeted. "My name is Dr. Adel, the director of Anti-Skullgirls Lab 8. I'm here to warn you girls that the new Skullgirl has been born and wants to destroy the world."

"Yeah, I've kinda figured that out." Lilith spoke as she rolled her eyes and crossed her arms over her chest.

"And what can we do to stop the Skullgirl?" Alice asked.

"Each of you are the descendants of the Skullgirl candidates few years ago." Dr. Adel explained as he pulled up picture information of the Skullgirl candidates. "Each girls were fighting for the Skull Heart, but each of them ended up either destroying it and wished on it."

The girls watched the computer as it showed an image of Filia winking with Samson crossing his arms.

"This is Filia and her hair parasite Samson." Dr. Adel stated. "She is Alice's ancestor and they both have hair parasites that are capable of performing mix ups that can keep their opponents on their toes as the hair parasites shifts into different shapes and sizes."

"That chunky schoolgirl with that scary-looking parasite is our ancestor?" Alex asked in exclaim. Then she let out a calm smile. "That's kinda cool!"

"Her life as a schoolgirl is really smiling to my life with Alex." Alice said as she cocked her head slightly.

"What are you trying to say?" Alex shouted.

"Moving on." Dr. Adel interrupted as he ignored the twin conservation between Alice and Alex. He stared at the computer as it showed an image of Cerebella at her circus performance with Vice-Versa. "This is Cerebella and she is Andrina's ancestor. They both are talented dynamic acrobats that has no trouble adjusting their body to suit Vice Versa's needs."

"She is amazing and gorgeous!" Andrina smiled cheerfully. "I'm so glad I've took her circus legacy as a sexy acrobat!" She looked up to notice Vice-Versa was feeling sad for his former partner and gave the hat a gentle pat. "It's okay, Vice-Versa. I'm here for you as always."

"And that's makes the two of you an formidable duo." Dr. Adel said with a grin. He turned back to the computer as it showed an image of scary-smiling Peacock with her carton cronies. "This is Peacock and she is Lauren's ancestor. They both has been infused with two synthetic parasites which has been granted some very unique abilities as a result in the lab."

"I've never thought that my cartoon-loving ancestor will be so awesome for real!" Lauren grinned. "But I'm glad that Avery is there for me."

"Avery?" Riley asked in confusion.

"Yeah, he's my synthetic parasite." Lauren replied. "But unlike other synthetic parasites, he has a free will."

"I've learned 'that' the hard way." Dr. Adel scoffed as she turned back to the computer and watched it showing an image of Parasoul in her Black Egret outfit with her troops. "This is Parasoul and she is Lila's ancestor. They both are very strategic charge fighter on the battlefield to can keep opponents at bay with their living weapon Krieg."

"She's very firm and elegant." Lila gushed. "I promise that I will fulfill her legacy as leader of the Black Egrets."

"Black Egrets?" Sarina asked as she lifted her eyebrow.

"They are the elite military troops." Lila replied. "They served me to protect the Canopy Kingdom and its people."

"And I'm proud the king developed the lab for the sake of his wife." Dr. Adel nodded his head. Once he turned back to the computer, the screen showed the image of Ms. Fortune with her head off as if it was propelling blood in the air. "This is Ms. Nadira Fortune and she is Molly's ancestor. They both are the combination of a "puppet" and "rushdown" catgirl thanks to the Life Gem that Nadira shallowed in her days."

"What is an Life Gem?" Lilith asked.

"It's a very rare gem that allows the owner to live forever." Molly stated. "Thanks to my ancestor, I've given an gift to live forever with my whole family."

"Remarkable." Dr. Adel muttered as he turned back to the computer and watched once it showed the image of Painwheel running in rage and anger. "This is Painwheel and she is Riley's ancestor. They both use their Buer Drive blade as well as confronting foes with spikes that eject painfully from their body."

"She must be a poor girl who just want an normal life like I did." Riley said softly.

"Do you still want to?" Sarina asked.

"Not anymore." Riley replied.

"It's good to focus on your true and free destiny." Dr. Adel nodded as he turned back to the computer once it showed an image of Valentine holding her bonesaw in combat. "This is Valentine and she is Sarina's ancestor. They both use lab tools as ninja weapons in deadly combat."

"Not only she's deadly, but she is so brutal." Sarina gushed. "I like it!"

"That makes two of us." Lauren swooned playfully. "Hello, nurse!"

"Uh-huh." Dr. Adel scoffed as he turned back to the computer and watched it showing an image of Double. "And this is Double and she is Lilith's ancestor. They both are capable of transforming their nightmarish form into that of any person they contact."

"And now, I live with her inside the Grand Cathedral." Lilith added as she crossed her arms. "Unaware of the whole thing."

"Ladies, far as your concern, all of you are the only one that have the maximum strength and skills to stop the Skullgirl." Dr. Adel spoke in a serious tone. "What do you say?"

"We're in!" Alex, Lauren, Molly, and Lilith agreed in excitement.

"Me two." Sarina concurred.

"Me three." Lila nodded.

"If Alex's in, then I'm in." Alice sighed.

"Count me in." Andrina winked.

"Since I'm the last one, I'm ready to fight." Riley shrugged.

"Then we must act fast." Dr. Adel nodded as he glanced back at the computer. "Because only three of our enemies are routing the city as we know."

"Let's go!" Lauren smiled.

With that, the girls followed Lauren on her car and drove off to their destination.