Desmond Miles POV

I relived the life of Jacob Frye and Evie Frye in the Animus Omega at Abstergo Industries Philadelphia facility. In 1868, Henry Green—born Jayadeep Mir, the son of Indian Assassin, Arbaaz Mir—writes to the Assassin Brotherhood begging for aid. Green explains that the Brotherhood in London has fallen, leaving the city at the mercy of Templar Grand Master Crawford Starrick, who controls both London's industry and the criminal underworld. Starrick plans to use his wealth and influence to seize control of Britain and, through its holdings, the world. Outside of London, the Frye twins begin their work, with Jacob assassinating a corrupt factory boss, Rupert Ferris. Evie infiltrates a lab owned and run by and Templar occultist Lucy Thorne. Inside, Evie finds Brewster experimenting on a Piece of Eden and assassinates him. Brewster tells Evie that Starrick knows of a second, more powerful Piece of Eden when the first Piece of Eden becomes unstable and explodes, forcing Evie to flee. With their missions successful, the Frye twins decide to disobey orders from the Brotherhood and head for London.

In the past, the Frye twins arrive in London and meet with Henry Green, who reveals himself as an old acquaintance of their father. They have different ideas on how to liberate London: Jacob advocates that they take the fight straight to the Templars, while Evie advises they find the Piece of Eden first. They agree to begin liberating the various boroughs of London by defeating Templar-controlled gangs, sabotaging Templar-controlled businesses, assassinating high ranking Templars, and building up their own criminal gang called the Rooks. Along the way, they enlist the help of various people including Charles Dickens, Frederick Abberline, Alexander Graham Bell, Florence Nightingale, Edward Hodson Bayley, and a young Arthur Conan Doyle.

Jacob decides to investigate the mysterious and addictive "Soothing Syrup" that Starrick has been distributing all across London and is slowly poisoning the populace. He meets Charles Darwin, who is also investigating the syrup. Together, they destroy the factory producing the syrup and interrogate. Owen informs them that has been manufacturing the syrup. Jacob and Darwin then head to Lambeth Asylum, where Jacob assassinates Elliotson. Next, Jacob decides to assist Pearl Attaway, one of Starrick's competitors in the omnibus business. He sabotages Starrick's omnibus company and assassinates its boss, Malcolm Milner. However, Milner warns Jacob that Starrick and Attaway are cousins and that Attaway is a Templar. In response, Jacob assassinates Attaway. Jacob then learns of Templar banker Philip Twopenny's plot to rob them and steal its gold reserve. With Abberline's assistance, Jacob breaks into the Bank and assassinates Twopenny. Jacob then heads to Parliament to prevent a Templar plot to assassinate British Prime Minister Benjamin Disraeli, who is pushing to pass the Corrupt Practices Act. Jacob assassinates the mastermind, the Earl of Cardigan.

Jacob is then contacted by Maxwell Roth, the leader of the Templar-controlled gang, the Blighters. Roth offers to betray the Templars and form an alliance with the Rooks, which Jacob accepts. After assisting Roth in several missions against Starrick, Jacob breaks off the alliance when Roth tricks him into bombing a building full of child laborers. Jacob infiltrates Roth's at the Alhambra Theatre headquarters and assassinates Roth.

Meanwhile, Evie looks for the Piece of Eden, managing to steal an Assassin journal from Thorne. Analyzing the journal, Evie discovers that it refers to the Shroud of Eden, a Piece of Eden that can heal any injury, including old age, and makes the wearer immortal. She follows the trail of clues underneath the mansion of, where she finds a map detailing the locations of all of the Assassin vaults hidden in London. Her search leads her to famous landmarks such as the Monument to the Great Fire of London and St Paul's Cathedral. She obtains the key needed to access the Shroud's vault, but it is stolen by Thorne. Evie then heads to the, where the vault is located and assassinates Thorne. Thorne explains the Shroud is not in the Tower and claims that the Assassins have no idea of the true power of the Shroud before dying. Green believes the real vault is hidden in Buckingham Palace and enlists the aid of the Maharajah Duleep Singh to find the schematics for the building. Unfortunately, the Templars manage to seize the schematics first. In addition to her hunt for the Shroud, Evie also helps correct the unintended consequences Jacob's assassinations have caused such as medicine shortages and currency inflation.

With all of his lieutenants dead, Starrick moves to retrieve the Shroud personally. Throughout the latter part of the game, the twins' different views on approaching their time in London come to a boiling point when Jacob and Evie get into an argument over Jacob's recklessness and Evie's apparent inaction. Henry warns the Frye twins that Starrick plans to break into Buckingham Palace, steal the Shroud, and kill all of Britain's heads of church and state. The Frye twins agree to work together to stop Starrick before going their separate ways. The twins infiltrate a ball being held at the palace, where they meet Queen Victoria and Starrick himself. Starrick beats them to the vault and obtains the Shroud for himself, which grants him superhuman strength and regeneration from wounds. Working together, the Frye twins and Green manage to defeat and kill Starrick. After the battle, Jacob and Evie return the Shroud to the vault, reconcile their differences, and agree to continue working together. In recognition of their deeds, Queen Victoria knights the Frye twins and Green.

The Frye twins are approached by Henry Raymond, a writer penny dreadful, as well as his follower, a young Arthur Conan Doyle. They team up to investigate a series of murders across London, visiting crime scenes, gathering evidence, interviewing witnesses and suspects, and ultimately solving the crimes by accusing the true perpetrators.

The twins are eventually summoned to Buckingham Palace by Queen Victoria, in order to solve the murder of one of her guards. It turns out that it is, in fact, Raymond who has posed as a guard and faked his own death, so he may steal the Queen's Sceptre of the Dove by observing up close the combination to the safe that holds it. He has also left a series of false clues suggesting a bomb threat in the palace in order to cover his escape. However, Doyle has already uncovered his plan and set out to stop him, but is held hostage on the palace roof in the process. One of the Fryes manages to distract Raymond while the other sneaks up and kills him, saving Doyle and recovering the Sceptre. In the aftermath, the Fryes encourage Doyle to try his own hand at writing detective fiction.

After an unpleasant conversation with Duleep Singh regarding his lack of commitment to the people of India, Henry Green decides to enlist the help of the Frye twins so the Maharaja could be persuaded to reclaim his birthright. The twins manage to recover Singh's letters to his mother, which were previously intercepted by the British Indies Company (B.I.C.) in order to stop any correspondence. This finally convinces Singh that he should take action.

Singh orders the twins to locate and recover the stolen Punjabi gold, before arranging for transports to ship the gold back to India. He then proposes to recover the Koh-i -Noor, a diamond in the possession of Queen Victoria, currently kept in the Tower of London. The twins accomplish the goal by infiltrating a gala held at that location; however Henry shows that the diamond they recover is a replica, and the true Koh-i-Noor is in fact in the safe hands of the British Assassins, thanks to Jayadeep's father Arbaaz Mir handing over the diamond to Jacob and Evie's father Ethan Frye.

The twins head to a B.I.C. factory and put an end to the company's production of biological weapons. In the process, they also discover that Brinley Ellsworth, a close friend of Singh's, is in fact behind the plots against the Maharaja. Singh arranges for a meeting where he confronts Ellsworth. With Evie's help, Ellsworth is subdued. However, as Evie prepares to execute Ellsworth, she is stopped by Singh, who chooses to exercise mercy. Singh thanks the twins for their contribution before parting ways with them, continuing to fight for his heritage.

The story begins in 1888, 20 years after the main game, with the Assassin Jacob Frye meeting Mr. Weaversbrook, who is warned not to publish 's Jack letters as he wants to spread fear in London. He then receives word of another murder and goes to investigate. Jacob goes after the Ripper, who begins following him before eventually attacking. As the Ripper pursues Jacob, it is revealed that he knows Jacob personally, and possesses Assassin abilities. After escaping, Jacob reaches his lodgings, but the Ripper arrives and attacks again, with Jacob seemingly being killed.

Following the incident, Jacob's sister Evie Frye arrives from India, having been summoned by Jacob sometime before, where she is greeted by police inspector Frederick Abberline, who informs her that Jacob is missing and presumed dead. He also tells her that she may be the last Assassin remaining in London, and the only one capable of stopping the Ripper. After finding Jacob's lodgings, Evie takes some non-lethal fear tools used by the Indian Brotherhood. She also deduces that the Ripper is, in fact, one of Jacob's Assassin Initiates. Afterward, she kills the Ripper's lieutenants who have been aiding in his crimes and frees a number of prisoners he had been keeping hostage. All the while, the Ripper stalks Evie.

With more murders occurring, Evie is pressured to find the Ripper quickly; after the Ripper's final murder, Abberline makes it plain unless she delivers the Ripper, he will not be able to stop her from being arrested for the crimes – Evie vows to stop the Ripper or die to try. She re-examines all of his old crime scenes and finds hidden clues that lead to him, as well as learning the fact that all of the women he murdered were Assassins. She later finds a message left by the Ripper, which reveals that he never forgave Jacob for failing to protect his mother from being killed by Starrick's men. Evie deduces that the Ripper is waiting for her at Lambeth Asylum, where he was imprisoned before Jacob recruited him into the Brotherhood. Meanwhile, the Ripper returns to the Asylum and murders his former tormentors. Evie arrives shortly after the Ripper and kills him in battle. Afterward, she finds an imprisoned but still alive Jacob. With the Ripper dead, Abberline agrees to cover up the Ripper's identity as an Assassin in order to protect the Brotherhood.

In 1916, at the behest of Winston Churchill, Lydia infiltrated and eliminated a German Templar spy facility in Tower Bridge. She later sought out, on his request, the rest of the group and their Sage leader that he deemed to be a pressing matter of national security which he was unable to broach with the government as he lacked strong enough evidence. In exchange for her services, Churchill promised Lydia that he would do what he could for the enfranchisement of women when he had returned to parliament after the War.

Lydia later conquers every single Templar-infested area, killing German spies and Templar captains, finally flushing the Sage out of his hiding place and killing him.

I recovered the Precursor box as well as the Shroud of Eden 2 and the Koh-i-Noor. Afterward, I shot Violet da Costa, Juhani Otso Berg, Isabelle Ardant, and Alvaro Gramatica. Later, I detonated the precursor DNA, all the clones of John Standish, and the clone of Juno along with the facility in Shanghai. Which lead to the end to the Phoenix Project and the instruments of the first Will.