Shinobu has grown and matured since No More Heroes 2. Wiser and kinder, she takes it upon herself to become a vigilante...However, one night, fate throws her for a loop when she witnesses a little boy abused by his family...

Margaret Moonlight is a vigilante that hunts down sinners and brings them to justice. She has had her eye on Vernon Dursley for a long time now, and when she sees the way he treats his nephew, she not only gets her opportunity to have him locked up...She also gets the rewarding position of mother.

Upon retiring from head of the UAA, Sylvia Christel defends a little boy from an assassin and takes him home, deciding to raise him herself. However, she unknowingly killed an assassin ranked in the UAA. As a result, she is now forced to climb the rankings...