Characters on Mega Man's side:
Nick Fury, Mega Man, Eddie, Rush, Darci Roll, Supergirl, Black Panther, Wolverine, Storm, Shadowcat, Rogue, Professor X, Ice Guy, Phoenix/Jean Grey, Beast, Cyclops, Angel, Nightcrawler, Mr. Fantastic, Invisible Woman, Harley Quinn, Deadpool, Cat Woman, The Thing, Human Torch, Spider-Man, Iron Man, War Machine, The Penguin, Cyborg, Captain America, Batman, Black Canary, Robin, Black Widow and Hawkeye
Characters on Wily's side:
Dr Wily, Proto Man, Lex Luthor, Mercy Graves, Callisto, Pyro, Magneto, Lady Deathstrike, Dr. Doom, Electro, Green Goblin, Dr. Octopus, Bane, Dive Man (defeated), Drill Man (defeated), Toad Man (defeated), Bright Man (defeated), Pharaoh Man (defeated), Ring Man (defeated), Dust Man (defeated), Skull Man (defeated), Twin Robots, Stone Man (defeated), Charge Man (defeated), Wave Man (defeated), Star Man (defeated), Gravity Man (defeated), Gyro Man (defeated), Crystal Man (defeated), Napalm Man (defeated), Dark Man 1 (defeated), Dark Man 2 (defeated), Dark Man 3, Dark Man 4, three of ED-209s (two of three defeated) and two of Rolling Drills (both defeated), plus Robo-Spider
Robo-animals:
Robots of Prehistoric and modern animals:
Lepictidium (spy-robots, not described), Gastornis, Ambulocetus, Propalaeotherium, Godinotia, Titanomyrma (spy-robots, not described), Creodonta, Kentisuchus, Basilosaurus, Andrewsarchus, Embolotherium, Dorudon, Moeritherium, Apidium, Physogaleus, Arsinotherium, Indricotherium, Hyaenodon, Entelodon, Chalicotherium, Cynodictis, Megalodon, Odobenocetops, Cinereous Vultures, Australopithecus, Dinofelis, Deinotherium, Ancylotherium, Warthog, White Rhinoceros, White Backed Vultures, Ostriches, Paranthropus Boisei, Homo Habilis, Homo Rudolfensis, Black Eagle, Crocodile, Zebra Finch (spy-robots, not described), Lion, Impala, Vultures, African Bush Elephant, Swallows (spy-robots, not described), Tarantula-spider (spy-robots, not described), Giraffe, Baboon, Gigantopithecus, Smilodon, Phorusrhachos, Macrauchenia, Megatherium, Doedicurus, Wolly Mammoth, Megaloceros, Wolly Rhinoceros, Cave Lions, Saiga Antelope, Grey Wolves and European Bison
Other hostile robots:
Twin Robots, an ED-209, Dark Man 3, Dark Man 4, Cavemen and a Robo-Spider (the last one)
Other villains:
Lex Luthor, Mercy Graves, Callisto, Pyro, Magneto, Lady Deathstrike, Dr. Doom, Electro, Green Goblin, Dr. Octopus, Bane, Proto Man and Dr Wily
Robo-Spider (1st August 2066) (Nearly a year after the 1st part of Mega Man 5)
Now, those heroes, such as Mega Man, Invisible Woman, Darci Roll, Spider-Man, Rush, Eddie, Supergirl, Batman, Iron Man, Nick Fury, Captain America and Cyborg, goes on by two different marine-ships. However, they entered so called "Mammalbot Park". They also meets mechanical sea monsters such as Basilosaurus, Dorudon, Physogaleus, Odobenocetops, Megalodon, Kentisuchus and Ambulocetus. Iron Man and Mega Man comes out of these ships, to first scare away Kentisuchus, Odobenocetops, Physogaleus, Ambulocetus and Dorudon. Then they defeats other hostile robot sea monsters.
After defeating those robots, Mega Man and Iron Man comes back to these ships, and continues on to the land. When they already are on land, they unexpectedly meets three Sniper Joes and two Metbots, which those heroes easily defeats. Then an ED-209 comes to block their way from continuing on.
"You're not allowed to pass further through the land" as the ED-209 says.
"Ok, it's showtime!" as Captain America shouts.
Those heroes immediately defeats this ED-209 robot. After defeating this ED-209 robot, they're continuing through the new forest. When those heroes entered there, they meets several mechanical races of prehistoric animals, such as Gastornis, Propalaeotherium, Godinotia, Creodonta, Andrewsarchus, Embolotherium, Moeritherium, Apidium and Arsinotherium. First they scares away Propalaeotherium, Godinotia, Creodonta and Apidium. However, they confronts and defeats the other robot animals.
After defeating those robot animals, those heroes meets Twin Robots.
"Stop, don't go anywhere!" as those robots says.
"Ok, I'll do it alone" as Iron Man says.
"Stop, don't do it. Are you crazy??" as Invisible Woman says.
"I know what I'm doing" as Iron Man responds.
Now, the first Twin Robot tries to aim on Iron Man. But when Iron Man hides behind the second one, the first one wouldn't shoot, because of the second one is on the way. Iron Man takes down the second one, hiding very good and defeats the first one too. Now Iron Man comes back to those heroes, to continue forward.
While continuing through the forest, those heroes meets mechanical races of prehistoric animals, such as Indricotherium, Hyaenodon, Entelodon, Chalicotherium and Bear-Dog. Those heroes defeats these hostile robots, then they continues to go on through the Park. After defeating those robots, those heroes meets Dark Man 3, and Dark Man 4.
"You're not allowed to pass further away" as Dark Man 3 says.
"Otherwise we'll burn you" as Dark Man 4 says.
"We'll dare to make a trouble" as Mega Man says.
Mega Man along with Captain America and Cyborg defeats Dark Man 3, while Supergirl and Iron Man together defeats Dark Man 4.
After defeating those Dark Men robots, those heroes meets several mechanical races of prehistoric and modern animals, such as Cinereous Vultures, Australopithecus, Dinofelis, Deinotherium, Ancylotherium, Warthog, White Rhinoceros, White Backed Vultures, Ostriches, Paranthropus Boisei, Homo Habilis, Homo Rudolfensis, Black Eagle, Crocodile, Lion, Impala, Baboon, Giraffe, Vultures and African Bush Elephant. Those heroes confronts and defeats those robot animals.
After defeating those robots, they meets a gigantic primate, Gigantopithecus. Then they confronts and defeats that robot.
After defeating robot Gigantopithecus, those heroes went on to a place where they meets mechanical races of prehistoric animals, such as Smilodon, Phorusrhachos, Macrauchenia, Megatherium and Doedicurus, plus those first robot Cavemen. Those heroes confronts and defeats those robots, then continues on to the Ice Age Place. The Ice Age Place is the last part Mammalbot Park.
In the Ice Age Park, those heroes meets mechanical races of prehistoric animals, such as Wolly Mammoth, Megaloceros, Wolly Rhinoceros, Cave Lions, Saiga Antelope, Grey Wolves and European Bison, plus those last robot Cavemen. Those heroes confronts and defeats these hostile robots, then forces those robot Cavemen to surrender.
After defeating all those animal and Cavemen robots, those heroes meets other ones, they are Black Panther, Wolverine, Storm, Shadowcat, Rogue, Professor X, Ice Guy, Phoenix/Jean Grey, Beast, Cyclops, Angel, Nightcrawler, Mr. Fantastic, Harley Quinn, Deadpool, Cat Woman, The Thing, Human Torch, War Machine, The Penguin, Black Canary, Robin, Black Widow and Hawkeye.
Outside the Robosaur styled-park, Wily appears along with Proto Man and his supervillains, Callisto, Pyro, Magneto, Lady Deathstrike, Dr. Doom, Electro, Green Goblin, Dr. Octopus, Bane, Lex Luthor and Mercy Graves, plus also with the Robo-Spider. Those heroes defeats all of the supervillains and Proto Man, except Wily, who orders his gigantic Robo-Spider to attack. Mega Man confronts it alone, secretly obtain this web. He figures out how to destroy the Robo-Spider, by using some form of electric stuffs. Then it causes the Robo-Spider to collapse.
Wily and his team gives up once again.
Now, Mega Man along with other heroes returns back to their league's home, they celebrates their happiness.
Mega Man 5: Robo-Spider; THE END