In another white room on another floor , Desmond is also being forced into an Animus who was angry with his captors telling them " I am only a bartender what do you want with me ? "
" We know your history Mr. Miles and we also have one of your distant relatives a female that you Assassins fight soo hard to protect. Help us or like her we will put you into a coma and continue then when we are done leave you to die. "
Given no other choice Desmond gets into the Animus. From that point on, Desmond was made to explore through the genetic memories of Altaïr Ibn-La'Ahad – a Syrian man who was a member of the Assassin Order during the High Middle Ages. For several days, Desmond did so under the Templar's watchful eye, but during the nights, he was anonymously given a code that allowed him to open the door to the bedroom provided for him, which granted him the ability to explore the laboratory that he was being held in. As he was presented with the opportunity, Desmond used it to access the Animus' computer, and, after he had stolen Warren's access pen, gain access to Warren's personal computer as well.

Through reading private emails, Desmond learned much about Abstergo, as well as the Animus subject that had preceded him, Subject 16, who was noted to have suffered from the "Bleeding Effect", a drastic symptom induced due to prolonged exposure to the Animus. Eventually, on Lucy's prompting, the Assassins attempted to liberate him and Sakura, with the gunfire of their assault being heard through an intercom device. After the rescue attempt failed, Warren boasted that those Assassins were, in fact, the last of the group Desmond had escaped from, and that their desert hideout had been previously destroyed. When Warren left, however, Lucy reassured Desmond that that compound had been found deserted and that his parents had "probably escaped". During this discussion, Lucy revealed herself to be an undercover member of the Assassins.
Subsequently, Desmond eventually achieved Warren's goal and discovered the locations of numerous Pieces of Eden. Following this, three executives of Abstergo Industries ordered for Desmond's execution, but his life was saved by Lucy's intervention, as she asked them to keep Desmond alive in order to re-access his memories if the executives left, Desmond realized that he was able to use "Eagle Vision", an ability that he shared with Altaïr, which he had picked up through the Bleeding Effect. Using it for the first time, he perceived a strong interpretation of Warren and Lucy's true allegiances, as well as countless scrawled messages and puzzles on the walls and floor of the main laboratory and his own quarters.

After Desmond spent some time alone in the laboratory, Lucy suddenly returned, covered in blood and demanding that Desmond was to get into the Animus before Abstergo discovered what she had done. Desmond, rather confused, agreed to her request and managed to synchronize himself with the closest genetic match between him and Subject 16, which was recorded on the Animus' memory core. Inside the Animus, Desmond experienced the birth of another of his ancestors, Ezio Auditore da Firenze, before he was pulled out abruptly, so that the two could both make their escape.

During the escape Desmond stops Lucy " there is another we must take with us a distant relative of mine 'Sakura.' "
The Assassins had fought to protect the members of the Hayabusa clan and also serve as their allies throughout the ages.
" I see so we must do this quickly then Desmond. " as her response.
Sakura's parents died when she was very young and her adoptive family was killed by the Templars , but they arranged the family of Desmond Miles to be her godparent. Desmond then responds " since no one else is here this job will be mine , so where is she ? "
Lucy then accesses another terminal to find out the location where they were keeping Sakura and finding it heavily guarded. " its impossible if we go we will get caught " Lucy responds
" risky or not we still have to go. " Desmond responds.

Finding no other way to convince Desmond otherwise she agrees to help him rescue the other subject captured by Abstergo. The pair were quickly spotted by Abstergo's security, though Lucy was able to react fast enough to lock a door and keep the guards from reaching them. Unfortunately, they were forced into an engagement with two more security personnel, who Lucy promptly dealt with, astonishing Desmond and allowing them to continue undetected. The two soon entered an elevator which lead to the top floor where they were keeping subject Alpha.

Desmond finds Sakura strapped to an Animus surrounded by four guards. Not willing to see her in anymore pain he rushes to her aid , while calling for Lucy to aid him. With the help of Lucy Desmond proceeded to pummel the guards incapacitating them. After they are finished Desmond ran over to Sakura who is still connected to the Animus and finds her unresponsive when Lucy deactivates it.
" I am so sorry , I could not protect you " Desmond responds while holding back tears.
Lucy then puts a hand on Desmond's shoulder and talks to him" Desmond we must go now. "
" Yes "
Desmond responds as he picks up Sakura putting her on his back, then turning to Lucy " lead the way. "

As Desmond followed Lucy through the maze-like walkways between the blocks, in order to avoid the guard patrols, they eventually reached the back of the room. Lucy attempted to enter a code into the locked door of another elevator, but it was rejected, prompting Desmond to instinctively call upon his recently acquired Eagle Vision. Seeing the buttons necessary for the code, Desmond successfully unlocked the door on his third try. However, once they had both reached the car park underneath the building, They were then halted by more of Abstergo's guards, but were able to defeat them and escape.