Sakura remains unconscious while she was taken to the Assassins' hideout in Rome by Desmond. Upon arrival he is introduced to two more Assassins Shaun Hastings and Rebecca Crane , who had previously worked with Lucy. The other Assassins take note of the 18 year old female that is being carried in Desmond's arms. Shaun then spoke up " well who is that person ? "
" Her name is Sakura Hayabusa , a member of my extended family and a distant relative of me from my mother's side. My family is her Godparent. Like me she was also forced into an Animus by Abstergo. "
Lucy then tells Desmond that they will like him to be an Assassin as well , telling him that they are currently losing the war. Desmond wonders how this can be achieved when it takes months or even years of training.
Lucy then explains through the 'bleeding effect' the skills can be achieved in a matter of days. Doing so required him to learn through the ancestor that he had matched up with through Subject 16, being Ezio Auditore, and Desmond agreed to help almost immediately, which surprised Lucy.
" They kidnapped me and Sakura , hurt my family this is more than enough of a reason for me to do this. " Desmond replies when he as asked to become an assassin. After this, he was quickly plugged into the Animus 2.0, where he began to relive Ezio's memories.

Ezio finds it a bit hard to communicate with Izumi due to a language barrier. He is Italian and she is Japanese , after several days they are finally able to talk to each other , though a bit shaky at best. Ezio's father and brothers were killed when he was 17 and he was forced to leave their birthplace with his mother and sister. Taking refuge in another town , there Ezio learns of his heritage , inherits his father's white assassin's robes , begins his his Assassin training and set about on his quest for vengeance against the Templar Order, and their Grand Master, Rodrigo Borgia, who had ordered the execution of his kin.
Ezio had just gotten the skills and the hidden blade needed to carry out his plans and was on his way to do it when the commotion started.
" There is nothing I can do at this time , if you need something done let me come with you maybe I can help ? "
Reluctantly Ezio allows Izumi to join him but tells her to stay out of sight as he proceeded to kill Uberto Alberti.
" Ok " Izumi responds. Ezio sought out and assassinated Uberto in the courtyard of the Basilica di Santa Croce, during Andrea del Verrocchio's latest exhibit. Once he had made his way into the courtyard, Ezio attacked Uberto in a frenzied rage, stabbing him multiple times in the chest, before proudly proclaiming the survival of the Auditore family, through him.
After killing Uberto, Ezio took the documents that the Gonfaloniere had concealed from him and his father, as well as a letter from Uberto meant for his wife and son. Ezio decided that he would see to it that she received the letter, not wanting to sink to Uberto's level.
After the act Ezio became the most wanted man in Florence , Ezio then seeks aid from Paola and letting his family know about going to Spain , he then talks to Izumi " If you have no where else to go come with me. "
With that said Izumi joins Ezio on his quest " I will was her response. "

Sakura awakens and looks around confused " where am I ? "
" you are safe back with the assassins , Desmond brought you here " said a voice. Sakura turns to look at the owner of the voice a rather older looking man.
" I am William Miles , Desmond's father and your godfather you may not remember this since you were still very young but your true parents had arranged this in case something were to happen to your foster parents and them , I am sure Abstergo said something about your background having ninja and assassin blood. "
" Yes, I was so scared , what am I ? , what did they mean when they said that I had ninja and assassin blood ? Then they forced me into a machine and i saw someone named Izumi Hayabusa... I am just a civilian from Japan. "
" Yes , that is the truth you are indeed a descendant of the Hayabusa clan a family of Assassins and Ninjas in the federal era of Japan. For many years we assassins had been working with that clan as allies and protect the members of the clan at all costs , the clan protects the apples of eden more so their shrine maidens do and you are the direct descendant of one of their master ninjas. "
Sakura turns away " I do not understand why this has been kept a secret from me , but seeing all these things happen to me i want to learn more about myself , who am i , why the assassins protect me at all costs let me use the Animus as well I need to know. "
" you are certain then "
" Yes "
" I thought you will say this " William responds , he then leads Sakura into another room with an Animus chair and directs Lucy to watch over her. Sakura returns to the life of one of her ancestors.

Ezio had left the city in hopes of going to Spain with his sister and mother stopping beforehand at the Auditore family's villa in Monteriggioni for shelter. As they neared the town, the three were accosted by Vieri de' Pazzi and his followers intending to take Ezio's life and kidnap the women for himself. Ezio fights off the attackers when Vieri then gives his allies another order to just kill everyone. Izumi then throws one of her kunai at Vieri's hand , then drawing her ninjato and dispatches the attackers alongside Ezio.
The fight drew the attention of Ezio's uncle, Mario Auditore after the brawl Ezio attempts to return the sword back to Mario.
" no , keep it " Mario responds and Ezio then gives him a weird look.
" You don't remember ? Its me Mario. "
" Uncle Mario ? "
" its been a long time nephew , far too long "
Mario then takes note of the other person standing near Ezio wearing a black assassin robe " and who is that person standing next to you ? "
" Her name is Izumi Hayabusa. "
" I herd about what happened , come let's get you all away from here " gesturing for the group to follow leading them to the family villa. As they walk Mario talks to Ezio about what has happened. Most of it was still confusing to Ezio , Mario then tells his nephew not to worry and everything will make sense soon. Mario begins to explain the existence of Assassins in an obvious attempt to induct him into the Order. He spent some time teaching Ezio swordplay telling him its not for his uncle's sake but rather give him the skills he needed to defend his family revealing that if it was not for Izumi being there he could had been killed. Mario also revealed that many of Ezio's ancestors, including Giovanni, were members of the Assassin Order. However, Ezio refused his ancestry, wishing only to continue his journey to Spain for his mother's and sister's safety, with the help of the skills Mario had taught him.