All of the racers took off with a powerful, bluish colored Rocket Boost as they all headed down the cement paved road. The people on both sides of the race course cheered as the Super Mario Brothers took the lead, with the nefarious Wario Brothers directly behind. The racers grabbed all the item boxes, but mostly got fake Item Boxes, green-colored Koopa shells, and yellow bananas.

"All ready starting, and we're getting crap..." Wario growled as he shucked the banana behind him, smacking Waluigi on the head. "Wally! We have to get past those pesky plumbers! Fire off all of the artillery!"

Waluigi growled as he rubbed his head injury, muttering quietly to himself, "Yeah, yeah... I'll handle them, you tub of lard." He swerved to the right as he avoided being chomped by the chained Chain Chomp, who munched down on Baby Mario and Baby Luigi, causing the two babies to wail in pain.

Toad steered his kart as Toadette hung on tightly from behind, gawking at the gigantic Chain Chomp as Donkey Kong swerved in front of them. Toad tried swerving to the right, but Donkey Kong swerved to the right. Diddy Kong, who was in the back, smacked his butt at Toad as he laughed. Toad growled, and he slammed his foot on the pedal, causing his kart to crash into the DK Barrel. Donkey Kong and Diddy Kong yelped as they were pushed to the right, with Toad steering towards the left.

Grabbing the item box, Bowser received a Bowser Shell, which was exactly like the spiky green-colored shells on the backs of Bowser and Bowser Junior. Smirking evilly as he looked at the number of racers behind him, Bowser hung onto the railings of the Koopa King with his right hand as he chucked the Bowser Shell behind him, laughing as all of the five pairs of racers were bowled over. However, he took a closer glimpse of the scene as Toad and Toadette zipped out of the madness, with Toadette using the Golden Mushroom. Bowser snarled as he pounded his fists on the railing, switching places with Bowser Junior as he pressured the heavy vehicle, planning to ram the two mushroom kids off the road.

"Hold on, Junior," Bowser warned Bowser Jr. as he halted the vehicle, causing it to stop in its tracks. The two spiky Koopas were on the bridge of the circuit as Bowser started going backwards, planning to ram Toad and Toadette off. However, Toad spotted this, and he swerved to the left, passing Bowser and Bowser Jr. Sadly, however, one of the three hungry Piranha Plants snapped up his kart, causing Toad and Toadette to fall out, with Toadette screaming as she was dragged in the sand.

Meanwhile at the front, Waluigi tossed green shell after green shell at the wobbling Goombas, getting them out of the way as he switched with Wario. Wario fired away the Bo-Bomb in front of him, laughing as Mario and Luigi were blasted into the sky, allowing him and Waluigi to take the lead. However, the Wario Bros. were hit by a Yoshi Egg, resulting in two mushrooms and a green shell. Yoshi chuckled as he passed Wario, going over the mushroom on the left as Birdo tossed a banana right in front of the mushroom in the middle. Wario growled as he switched positions again with Waluigi, slamming his left foot on the pedal as the Wario Car started churning out more speed than drugs.

Toadette trembled as she was starting to et frightful, with Toad swerving amongst the bumps in the road. He glanced to his right, to see Bowser grinning at him like a great white shark. Toad growled as he tried ramming into the Koopa King, but instead, he and Toadette went spinning around as they were too light. Bowser Jr. chuckled as he held onto the railing, the Koopa King slamming back on the concrete road as he tossed a fake Item Box behind. Toad tried getting his kart back into action, but it was bowled over by a runaway Chain Chomp, which was controlled by Baby Luigi, with baby Mario steering their blue-colored carriage. Toad and Toadette screamed as they tried pushing on again, with King Boo passing them in the green-colored Piranha Pipes as Petey fired off a Blue Shell, watching the spiky winged blue shell soaring at an incredible speed.

The audience clamored for more racing action as things started to heat up in lap two. Yoshi and Birdo were blasted into the sky by a Blue Shell as they were rammed into the grass by Peach and Daisy, who were trying to make up for lost time. Daisy was in control of the wheel as Peach had their pink hearts surrounding the Heart Coach, with Koopa and Paratroopa approaching. Paratroopa fired two of his triple red shells at Peach, but the blonde princess grabbed the first red shell wile Daisy took the second. Koopa and Paratroopa screamed as they were attacked by their own shells, chucked back at them as Peach giggled, waving at them. However, the two princesses were pushed into the wall by Donkey Kong, who was using triple mushrooms to gain momentum. Diddy switched positions, firing behind him a gigantic banana that smiled. Mario and Luigi tried swerving to the left as the banana consumed the entire space within the tunnel, but they screamed in anger as they spun out of control. Wario and Waluigi laughed at them as they both chucked a Bo-Bomb behind, the chaos ensuing inside the tunnel being shocking as the Bo-Bomb blew up the racers, causing them to land flat on their faces. Toad and Toadette used the golden mushroom to zip by the madness, while Bowser and Bowser Jr. cruised through with a sparkling Star.

Third lap. Things were now beginning to become worse and worse as tension between Toad/Toadette and Bowser/Bowser Jr. became bigger. Bowser continued pushing Toad and Toadette to the side, while Bowser Jr. hung onto the one Star he had left. Sensing something, Bowser Jr. used the star immediately, causing Toad and Toadette to fall over. Just as Toadette was about to grab the Item Box, a lightning bolt electrocuted everyone, causing them to shrink and spin out of control as Petey Piranha and King Boo cackled, going over the tiny meadows as they used triple mushrooms. Toad regained control of the kart as Toadette grabbed the Item Box, getting a golden mushroom.

By this point in time, Bowser and Bowser Jr. were now in the lead, having taken care of Donkey Kong and Diddy Kong. The baby mario Brothers were coming in from the rear, but Bowser Jr. fired off several green Koopa shells, causing Baby Mario and Baby Luigi to spin out of control as they bumped into a Goomba. Bowser Jr. received a high five from Bowser as they went over the tiny bridge, landing roughly on the concrete as they passed the finish line, gaining firs place. Peach and Daisy were closing in at second, but at the last moment, Toad bowled them over with a Star as Toadette used her golden mushroom to its best potential as they snagged second place. Koopa Troopa and Paratroopa made up for lost time as they snagged in third, tossing back any remaining green and red shells they had behind them as Waluigi swerved around the projectiles, claiming fourth for himself and Wario. Peach and Daisy continued being screwed over as Mario and Luigi snagged the sixth position, using the fireballs they had in their hands, which caused Peach to rub her butt as the Heart Couch spun out of control. Petey Piranha and King Boo used another lightning bolt, laughing at Peach and Daisy as the bosses received seventh place, celebrating despite being so far behind. Peach and Daisy moaned as they weakly made it to the finish line, only to claim ninth place as Baby Mario and Baby Luigi used a last minute Chain Chomp to secure eight place. The audiences cheered for all the racers as they celebrated, or lamented, on their positions.

"My, my! What a spectacular race!" Lakitu exclaimed as his glasses were blown off his face, landing right back on it a few seconds later as he commented, "You guys did an excellent job out there! I can't wait to see what the next race will be like!"