Later Batman is researching where the Joker has taken Ms. Bellum. "I suppose that the crazy crime-clown has used Ms. Bellum as bait." Says Alfred walking into the lab. "Joker has taken Sara Bellum into a laboratory in Townsville." Says Batman. "And I assume that he must be acquainted with a villain here in this town." Says Alfred. "Well I am about to find out." Says Batman, hopping into the Batmobile. He starts to drive down to the Townsville laboratory.
Meanwhile in the Townsville laboratory, the Joker has tied up and gagged Ms. Bellum to a chair. "What do you think Bellum?" says the Joker, showing her the vile of Chemical X. "Once we insert this into our newest destructo-cannon, it will permanently wipe out this Powerpuff freaks once and for all." He then gives this to Mojo Jojo, who pours it into the cannon's tube. Mojo laughs and says, "And once those brats are out of the way, we'll finish off Batsy and take over the world." Harley removes the gag off of Ms. Bellum's mouth. "You sick, murderous, psychopath!" she yells, "You won't get away with this!" Harley immediately sticks the rag into Ms. Bellum's mouth.
Just then the Powerpuff girls crash through the roof. "Not so fast!" says Blossom. "Let Ms. Bellum go!" says Bubbles. "Or else we'll give out a knuckle sandwich." Says Buttercup. "Well well well," says the Joker giggling, "you're just in time to witness my most powerful new weapon." He reveals Mojo Jojo with the destructo-cannon. "Mojo Jojo!" says the Powerpuff girls. "I should've known that you would be working with this madman!" "Well you see," says Mojo, "he has brought a vile of Chemical X to eliminate you three. And check out my new cannon." The Powerpuff Girls are surprised by the looks of it. "What's with this new machine?" asks Buttercup. "I'm glad you asked." Says Mojo, "I have added half the vile of Chemical X and mixed it with a full vile of Antidote X. And with this, the laser will eliminate your powers and you three will be here no more. And after you three are out of the way, there will be no one to stop us from ruling the world with an iron fist." "Oh," says Buttercup, "I bet we can whoop you two before you can hit us with that thing." "Yeah!" says Bubbles.
"Oh we'll just have to see about that." Says the Joker, then he turns to Mojo and says, "I'll keep them busy while you try to take aim." Then the Joker turns to the girls and takes out three Joker razor cards. He throws them at them, who dodges them easily. The Joker laughs and then rushes over to fight the girls physically. He kicks Blossom, next he grabs Bubbles and throws her at Buttercup. "This will be a lot harder than you think!" says Buttercup as she flies over to pummel the Joker. She punches him, sending him flying at the wall. The Joker gets up and fights back. She grabs Buttercup and shocks her with the electric hand-buzzer. As he laughs and continues to fight off the Powerpuff trio, Mojo Jojo takes aim at them. He fires at the girls, but they easily dodge the blast, much to Joker's frustration. "What are you doing?!" he yells, "Hit them!" "Alright!" snaps Mojo, "Just distract them to the point of exhaustion."
Meanwhile, Harley Quinn is monitoring the lab's security cameras. Suddenly she sees Batman arrive in the building. She immediately informed the Joker via communicator through ear-piece. "Mr. J!" she says. "What is it Harley?" asks the Joker who is having fun taking down the Powerpuff Girls, "I am in the middle of fun." "But puddin'," says Harley, "Batman has entered the building." Mojo tries to take aim at the Powerpuff girls, but as soon as the laser fires, Batman jumps out and saves the girls from the laser. The laser ends up hitting the wall. After the smoke cleared, the Joker says, "Well well well, if it isn't old Batsy." "What are you planning Joker?" he asks. "What I am doing?" says the Joker, "I am luring you here to get destroyed." "Alright Joker and Mojo," says Blossom, "enough fooling around." "It's jail time." Says Buttercup. "For you." Says Bubbles. "Oh," says Mojo, "we'll like to see you try." He presses the laser button and the cannon starts to fire again. Batman and the girls dodge it again. "I'll hold off the cannon." Says Batman, "you go untie the hostage." As he distracts the Joker and Mojo Jojo, the Powerpuff girls fly over to help Ms. Bellum. Blossom uses her heat vision to melt the ropes off. "Thanks girls," says Ms. Bellum, "Now go help Batman." Batman is fighting the Joker, who delivers a punch. Batman dodges it and retaliates with a knockout punch, sending the Joker at the wall. As he approaches again, Mojo aims the cannon at him, but he throws a batarang at the control screen. This causes the cannon to malfunction. "Curse you Batbreath!" cries Mojo as Batman jumps onto the cannon and grabs him. He throws him at the Powerpuff Girls who pummel him to the wall. "Alright girls," says Blossom, "let's finish him off." But the Joker knocks them out with a crowbar. Batman tries to fight him, but gets knocked out as well. The Joker and Mojo Jojo run for it. "There heading for the exit." Says Batman. "We can't let them get away." Says Ms. Bellum. "Come on," says Blossom. Bubbles carries Ms. Bellum while Buttercup holds onto Batman.
The Joker, Harley Quinn, and Mojo Jojo run for the exit. Suddenly the wall behind them blows up. It is the Powerpuff girls, Batman, and Ms. Bellum. "Not so fast bad guys!" says the Powerpuff girls. "Oops," says the Joker, "I almost forgot something for you." He throws a bag of marbles at them before running out the exit with Harley and Mojo. "Aw come on!" says Buttercup, "Seriously?" The marbles start to explode one by one. "They're grenades!" says Batman. "Come on!" says Blossom. She leads them through the ceilings and towards the roof. "You might want to lower your heads for this." She says. She bursts through the roof with Bubbles, Buttercup, Batman, and Ms. Bellum following. They quickly land on safe ground right before the laboratory explodes. "Thanks Batman," says Blossom, "we couldn't have saved Ms. Bellum without your help." "I'm aware of that." Says Batman before jumping into his Batmobile and driving off.