Hudson Thames as Peter Parker / Spider-Man: A 15-year-old who gained spider-like abilities after being bitten by a radioactive spider.

Michael Keaton as Adrian Toomes / Vulture: A salvager-turned-arms-trafficker after his company is forced out of business. He uses a suit with mechanical wings forged from Chitauri technology. Toomes is revealed to be the father of Liz, Parker's love interest. Keaton reprises his role from Spider-Man: Homecoming (2017).

Walt Hadfield: An former architect, former chauffeur and bodyguard for Rhodes and Potts, and former head of security for Stark Industries.

Zendaya as Michelle "MJ" Jones: One of Parker's classmates, also known as "MJ". Zendaya reprises her role from previous MCU media.

Donald Glover as Aaron Davis: A criminal looking to purchase weapons from Toomes. Glover reprises his role from Spider-Man: Homecoming (2017).

Jacob Batalon as Ned Leeds: Parker's best friend and guy in the chair. Batalon reprises his role from previous MCU media.

Laura Harrier as Liz Toomes: A senior, Parker's love interest, and Toomes' daughter, with a "type-A" personality. Harrier reprises her role from previous MCU media.

Tony Revelori as Flash Thompson: Parker's rival and classmate. Revelori reprises his role from previous MCU media.

Bokeem Woodbine as Herman Schultz / Shocker: An accomplice of Toomes' who is the second person to use modified, vibro-blast shooting versions of Brock Rumlow's gauntlets. Woodbine reprises his role from Spider-Man: Homecoming (2017).

Tyne Daly as Anne Marie Hoag: The head of the U.S. Department of Damage Control. Daly reprises her role from Spider-Man: Homecoming (2017).

Marisa Tomei as May Parker: Peter's aunt.

Don Cheadle as James "Rhodey" Rhodes / Iron Man: Parker's co-mentor, a former officer in the U.S. Air Force, Potts' fiancée, and former co-CEO of Stark Industries.

Beth Hoyt as Pepper Potts / Iron Woman: Parker's co-mentor, Rhodes' fiancée and the former co-CEO of Stark Industries who pilots the Iron Woman armor.

Kerry Condon as F.R.I.D.A.Y.: Rhodes and Potts' artificial intelligence, who powers their suits.

Josh Keaton as Steve Rogers / Captain America: A fugitive Avenger and World War II veteran enhanced by an experimental serum that gave him abilities to the point of human peak physicality. He is on the run following the Avengers Civil War. He appears via archival footage and PSAs, which the classes watch, particularly for detention or PE.

Garcelle Beauvais as Doris Toomes: Adrian's wife and Liz's mother. Beauvais reprises her role from Spider-Man: Homecoming (2017).

Jennifer Connelly as Karen: The A.I. in Parker's suit. Connelly reprises her role from Spider-Man: Homecoming (2017).

Hemky Madera as Mr. Delmar: The owner of a local bodega. Madera reprises his role from previous MCU media.

Gary Weeks as Foster: A Damage Control agent. Weeks reprises his role from Spider-Man: Homecoming (2017).

Logan Marshall-Green as Jackson Brice / Shocker: An accomplice of Toomes, who uses modified, blast-shooting versions of Brock Rumlow's gauntlets. Marshall-Green reprises his role from Spider-Man: Homecoming (2017).

Michael Chernus as Phineas Mason / Tinkerer: Toomes' accomplice, who is an expert on technology. Chernus reprises his role from Spider-Man: Homecoming (2017).

Michael Mando as Mac Gargan: An accomplice to Toomes. Mando reprises his role from Spider-Man: Homecoming (2017).

Christopher Berry as Randy Vale: An accomplice to Tolmes. Berry reprises his role from Spider-Man: Homecoming (2017).

Kenneth Choi as Morita: The grandson of Jim Morita and principal of Midtown High. Choi reprises his role from Spider-Man: Homecoming (2017).

Hannibal Buress as Coach Wilson: The school's gym teacher. Buress reprises his role from previous MCU media.

Martin Starr as Mr. Harrington: A teacher and Academic Decathlon coach. Starr reprises his role from previous MCU media.

Selenis Leyva as Ms. Warren: Parker's physics teacher. Leyva reprises her role from Spider-Man: Homecoming (2017).

Tunde Adebimpe as Mr. Cobbwell: Parker's teacher. Adebimpe reprises his role from Spider-Man: Homecoming (2017).

John Penick as Mr. Hapgood: Parker's teacher. Penick reprises his role from Spider-Man: Homecoming (2017).

Isabella Amara as Sally Avril: Parker's classmate. Amara reprises her role from previous MCU media.

Jorge Lendeborg Jr. as Jason Ionello: A student at Midtown High, who does the news with Betty Brant. Lendeborg Jr. reprises her role from previous MCU media.

Josie Totah as Seymour O'Reilly: Parker's classmate. Totah reprises their role from previous MCU media.

Abraham Attah as Abraham Brown: Parker's classmate. Attah reprises his role from Spider-Man: Homecoming (2017).

Tiffany Espensen as Cindy Moon: One of Parker's classmates. Espensen reprises her role from previous MCU media.

Angourie Rice as Betty Brant: Parker's classmate and a student at Midtown High, who does the news with Jason. Rice reprises her role from previous MCU media.

Michael Barbieri as Charles Murphy: Parker's classmate. Barbieri reprises his role from Spider-Man: Homecoming (2017).

Ethan Dizon as Tiny McKeever: Parker's classmate. Dizon reprises his role from Spider-Man: Homecoming (2017).

Martha Kelly as tour guide: A tour guide for the Decathlon field trip. Kelly reprises her role from Spider-Man: Homecoming (2017).

Zach Cherry as Klev: A street vendor who asks Spider-Man to do a flip. Cherry reprises his role from previous MCU media.

Kirk Thatcher as Punk Rock Guy: A guy interested in punk rock.

Stan Lee as himself: A New York City resident named Gary.

Chris Hemsworth as Thor: An Avenger, Loki's adopted brother, and the king of Asgard, based on the Norse mythological deity of the same name.

Tom Hiddleston as Loki: Thor's adopted brother and the god of mischief, based on the Norse mythological deity of the same name.

Jeff Goldblum as En Dwi Gast / Grandmaster: The former ruler of Sakaar and owner of the Contest of Champions. Goldblum reprises his role from previous MCU media.

Okioma Akagha as Shuri: The princess of Wakanda and T'Challa's tech-savvy, helping Bucky recuperate with his brainwashing.

Sebastian Stan as Bucky Barnes / Winter Soldier: Rogers' childhood best friend and a World War II veteran, who was thought killed in action before re-emerging as a brainwashed super-assassin.